pVL1392andpVL1393aretransfervectorsdesignedforhigh-levelexpressionofaclonedgenedrivenbythestrongAutographacalifornicamultiplenucleopolyhedrovirus(AcMNPV)polyhedrinpromoter(pPolh).
Description:
pVL1392andpVL1393arepUC8basedplasmidscontainingtheAcMNPVpolyhedrinlocuslackingthemajorityofthepolyhedrinopenreADIngframe.Amultiplecloningsite(MCS)sequencehasbeeninserted37nucleotidesdownstreamfromtheoriginalpolyhedrinATGstartcodon,whichhasbeenmutatedtoATT.
pVL1392andpVL1393shareidenticalsequences,withtheexceptionthattheyeachhavethesameMCSinopposingorientations.AstartandstopcodonmustbeprovidedwiththegenethatistobeclonedintotheMCS.Formaximalrecombinantproteinexpression,thedistancebetweenthecloningsiteandtheATGstartoftheinsertedgeneshouldbekepttoaminimum,andshouldnotexceed100nucleotides.
Specification:
| MediaType | ESF921andESFAF |
| PlatformCompatibility | BestBac1.0andBestBac2.0 |
| Shippingcondition | Ambient |
| Storagecondition | 2-8°C,protectfromlight |
RecommendedStorageCondition
2–8ºCExpressionsystems
总部设在加利福尼亚州戴维斯的ExpressionSystems致力于向细胞培养和生物工业市场提供和服务创新的媒体配方、辅助产品和合同服务。
Our well-characterized, superior-performing, protein-free insect cell culture medium is recognized as the industry standard for high throughput gene expression.
品牌 | 货号 | 名称 | 规格 |
Expressionsystems | 96-001-01 | ESF 921 Insect Cell Culture Medium, | Protein Free1L Bottle |
Expressionsystems | 96-001-08 | ESF 921 Insect Cell Culture Medium, | Protein Free8L Media Transfer Bag |
Expressionsystems | 96-001-10 | ESF 921 Insect Cell Culture Medium | Protein Free10L Media Transfer Bag |
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