ExpressionSystems’ProductionBoostAdditive(PBA)isanutrientboostdesignedtoincreaseproteinexpressionbysupportingtheincreasedneedsofinsectcellsinfectedbyrecombinantbaculovirus.PBAisaserum-free,animal-freeformulationthatcomplementsESF921andESFAFinsectcellculturemediaandextendsthelateandvery-lateexpressionperiodofproductionbymaintaininghighcultureviABIlity.

Description:

ExpressionSystems’ProductionBoostAdditive(PBA)isanutrient
boostdesignedtoincreaseproteinexpressionbysupportingthe
increasedneedsofinsectcellsinfectedbyrecombinantbaculovirus.
PBAisaserum-free,animal-freeformulationthatcomplementsESF921
andESFAFinsectcellculturemediaandextendsthelateandvery-late
expressionperiodofproductionbymaintaininghighcultureviability.
PBAisacocktailofnutrientsdesignedforsupplementationatarateof
1–10%at6–36hourspostinfection.

• Serum-free,animal-freeformulationworksseamlesslywithESF921andESFAF
• Extendscultureperiodbymaintainingcellviability
• Increasesproteinexpression        
• Availablein100mLor1Lbottles—inquireforcustompackaging
• Instockforimmediateshipment

Fig.1      Fig.2

TniProcells(Fig.1)wereinfectedwitharecombinantbaculovirusatanMOIof1.PBAwasaddedat1%oftheculturevolume18hourspostinfection.Sf9cells(Fig.2)wereinfectedatanMOIof0.02.PBAwasaddedat1%or5%oftheculturevolume36hourspostinfection.Therecombinantproteinwassecretedintotheculturesupernatantandharvesteddaily.ProteinexpressionlevelsweredeterminedbyproteinspecificELISA.

Specification:

RecommendedStorageCondition

Expressionsystems

总部设在加利福尼亚州戴维斯的ExpressionSystems致力于向细胞培养和生物工业市场提供和服务创新的媒体配方、辅助产品和合同服务。

Our well-characterized, superior-performing, protein-free insect cell culture medium is recognized as the industry standard for high throughput gene expression.