ExpressionSystems’BaculovirusTiteringKitfacilitatesaccuratedeterminationofinfectiousbaculovirustitersinlessthan24hours.Theassayisbasedondetectionofthebaculovirusgp64fusionproteinwhichisexpressedonthesurfaceofinfectedinsectcellswithinsixhoursofinfection.Thekitcontainsgp64-PEantibodyandabaculoviruscontrolofknowntiter.
Description:
Stainingofcellswithafluorescentlylabeledanti-gp64antibodyallowsforidentificationofinfectedinsectcells.Byinoculatingcultureswithaseriesoflogdilutionsofvirus,andstainingthecultures18–20hourspostinoculation,theratioofinfectedtouninfectedinsectcellscanbedeterminedbyflowcytometry.Statisticalanalysisofthepercentageofinfectedcellsinthevirusdilutionseriesenablesaccurateinfectioustiterdetermination.
Alternativeassaysthatalsoclaimresultsinunder24hours(suchasparticlecountinginstrumentsandqPCR)areindeedfastbutdonotdelivertrueinfectioustitervalues.Incontrasttotheplaqueassay,theculturingconditionsemployedfortheflowcytometricassaycloselyreflectactualculturingconditionsutilizedforexpressioncultures,resultinginamorerelevanttitervalueinafractionofthetime.Providedaccesstoaflowcytometerequippedtohandle96wellplates,theflowcytometricassayiseasilyadaptedtohighthroughputprocesses.
- ReproducIBLeandaccuratetiterresultswithin24hours
- Measuresinfectiousvirus
- SUSPensionculturemethodologysimulatesactualproductionconditions
- WorkswithanyflowcytometercapableofreADIngsamplesfrom96-wellplates
- Flowcytometricdatacollectionremovestheoperatorerrorofcountingplaques
- gp64-PEantibodycanbeusedalonetomonitorinfectionkineticsofbaculovirusininsectcellculture
Contents:
gp64-PE(R-PHYCOERYTHRIN(R-PE)-CONJUGATEDMOUSEANTI-BACULOVIRUSMONOCLONALANTIBODY)
Clone:AcV1
AntibodyComposition:MouseIgG2A
StorageBuffer:Phosphatebufferedsaline(PBS)containing0.05%BSA
and0.09%sodiumazide.
RecombinantBaculovirusControl
BestBac2.0co-transfectedwithemptypVL1393producedinSf9cells
Specification:
| Mediatype | ESF921orESFAF |
| Platformcompatibility | Workswithinfectiousbaculovirusproducedbymostmethods |
| Shippingcondition | BaculovirusTiteringKitshipsoncoldpacks,gp64-PEshipsambient |
| Storagecondition | 2-8⁰C,protectfromlight |
| Use-bydate | SixmonthsfromDateofManufactureforBaculovirusControl |
RecommendedStorageCondition
2–8ºCExpressionsystems
总部设在加利福尼亚州戴维斯的ExpressionSystems致力于向细胞培养和生物工业市场提供和服务创新的媒体配方、辅助产品和合同服务。
Our well-characterized, superior-performing, protein-free insect cell culture medium is recognized as the industry standard for high throughput gene expression.
品牌 | 货号 | 名称 | 规格 |
Expressionsystems | 96-001-01 | ESF 921 Insect Cell Culture Medium, | Protein Free1L Bottle |
Expressionsystems | 96-001-08 | ESF 921 Insect Cell Culture Medium, | Protein Free8L Media Transfer Bag |
Expressionsystems | 96-001-10 | ESF 921 Insect Cell Culture Medium | Protein Free10L Media Transfer Bag |
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