ExpressionSystems’popularBestBacLinearizedBaculovirusDNAbackbonehasbeenengineeredtodeliverhighlyefficientrecombinationwithallpolyhedrongenelocusbasedtransfervectors.TheresultingvirusvectorprovidessuperiorstABIlityandpuritycomparedtoothersystems.BestBacisavailablewithadeletionorwild-typeattheV-cath/chiAregionandsoldindividuallyorinkits.

Description:

• UnmatchedproductivityandstabilityforBEVS
• Improvedproductionmethodologycanreduceneedforplaquepurification
• Produceshightitersof5E8to3E9IU/mlofrecombinantbaculovirusinP1stock
• GeneratesP1stockasquicklyasBac-to-Bac®andothersystems
• EachBestBacvialcontainsenoughmaterialforaminimumoffivecotransfections
• SuitableforotherexpressionsystemsincludingBacMam^TM

AvailableinTwoVersions

• BestBac1.0iswildtypeatthecathepsinandchitinase(v-cath/chiA)loci,preferredforsomeapplications.
• BestBac2.0isadeletionmutantofBestBac1.0.Thev-cath/chiAdeletionremovesapotentiallydetrimentalproteaseandmayreducecompetitionforresourcesforproteinsynthesisduringlategeneexpression.Thev-cath/chiAdeletioncanimprovetheintegrityofyourexpressedproteinresultinginasignificantincreaseoffunctionalproteinyield.

AlsoAvailableinaCotransfectionKit

Containseverythingyouneedtoperformcotransfectionswithyourtransfervector.Includedinthekitare:

• BestBacDNA
• pVL1393-lacZcontrolvector
• Transfectionmedium
• Expres²TRtransfectionreagent

Specification:

RecommendedStorageCondition

Expressionsystems

总部设在加利福尼亚州戴维斯的ExpressionSystems致力于向细胞培养和生物工业市场提供和服务创新的媒体配方、辅助产品和合同服务。

Our well-characterized, superior-performing, protein-free insect cell culture medium is recognized as the industry standard for high throughput gene expression.