ExpressionSystems’TransfectionMediumisdesignedtocomplementESF921andESFAFinsectcellculturemediafortheco-transfectionortransfectionstepinbaculovirusvectorproduction.TransfectionMediumisaserum-freeandanimal-freeformulationthatfacilitatesvectorDNAuptakebytheinsecthostcells.
Description:
ExpressionSystems’TransfectionMediumisdesignedtocomplementESF921andESFAFinsectcellculturemediaforthecotransfectionortransfectionstepinbaculovirusvectorproduction.TransfectionMediumisaserum-freeandanimal-freeformulationthatfacilitatesvectorDNAuptakebytheinsecthostcells.RealizehighertitersbyusingaformulationdesignedtoworkwithESF921andESFAF.
- Serum-free,animal-freeformulationworksseamlesslywithESF921andESFAF
- AppropriateforPEIorlipofectionmediatedtransfection
- OptimizedforusewithExpres2TRTransfectionReagent
- Increasesvirusvectorproductionandtransientexpression
- Availablein20mLor100mLbottles
- Instockforimmediateshipment
ExpressionSystems’TransfectionMediumwascomparedtooldertechnologythatusesGrace’smediumfortheperformanceofcotransfectiontoproducebaculovirusvectors.SerumFreeMediumwasincludedasacontrol.ResultingvirustitersfromthecotransfectionwereseverallogshigherusingTransfectionMediumascomparedtoothermedia.TransientexpressionwasalsodeterminedasameasureofDNAuptake.Again,TransfectionMediumresultedinsignificantlybetterresultswiththepercentageofgp64expressingcellsbeingatleasttwo-foldhigherthanothermedia.
Sf9cellsweregrowninSerumFreeMediumandplatedintoindividualwellsofadeepwellblockataconcentrationof2x106cellsper100μlperwell.DNAandalipofectionreagentwereincubatedtogetherin200μloftestmedia(eitherSerumFreeMedium,Grace’sMediumorExpressionSystems’TransfectionMedium)for30minutes.DNAmixturewasaddedtocellsandvolumewasbroughtupto1mlintestmedia.CellsUSPensionwasincubatedforfourhoursat27°Cwhileshaking.Attheendoftheincubation,thevolumeofeachwellwasbroughtupto4mlwithSerumFreeMediumandculturedforfourdays.DeterminationoftheP0(i.e.,productofthecotransfection)infectiousunits(IU)viraltiterofthesupernatantwasperformedusingthegp64flowcytometricmethod.Transientexpressionofgp64wasdeterminedfortheculturedcellsbystainingwithgp64-PE.
Specification:
| Mediatype | Serum-free,animal-freeliquid,1X |
| Shippingcondition | Ambient |
| Storagecondition | 2-8⁰C,protectfromlight |
| Use-bydate | Oneyearfromdateofmanufacture |
RecommendedStorageCondition
2–8ºCExpressionsystems
总部设在加利福尼亚州戴维斯的ExpressionSystems致力于向细胞培养和生物工业市场提供和服务创新的媒体配方、辅助产品和合同服务。
Our well-characterized, superior-performing, protein-free insect cell culture medium is recognized as the industry standard for high throughput gene expression.
品牌 | 货号 | 名称 | 规格 |
Expressionsystems | 96-001-01 | ESF 921 Insect Cell Culture Medium, | Protein Free1L Bottle |
Expressionsystems | 96-001-08 | ESF 921 Insect Cell Culture Medium, | Protein Free8L Media Transfer Bag |
Expressionsystems | 96-001-10 | ESF 921 Insect Cell Culture Medium | Protein Free10L Media Transfer Bag |
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